OBJECTIVES: Staphylococcus aureus (S. aureus) is a nosocomial bacterium that commonly causes several infectious diseases in humans and animals. S. aureus infectious diseases are difficult to treat due to their biofilm.

MATERIAL AND METHODS: In the present paper, we studied phenotypically biofilm eradication methods via three different methods, i.e., Congo red agar (CRA), tube method (TM), and tissue culture plate (TCP). The inhibition of Ag and Se nanoparticles was performed by well diffusion assay against clinically isolated biofilm-forming S. aureus strains.

RESULTS: All S. aureus bacterial strains were found to be non-biofilm producers using the CRA method, whereas biofilm formation was performed by another method, namely TM. A total of 13.89% strains were shown strongly positive, while 55.56% isolates were unable to produce biofilm. On the other hand, with TCP method, 33.33% of strains were found to be high biofilm producer, while 44.44% were found non-biofilm producer.

CONCLUSIONS: The results of this study provided evidence of high inhibition growth of three biofilm-forming S. aureus (SA12, S A15, S A32) in the presence of silver nanoparticles (AgNPs) at 50 μl, whereas selenium nanoparticles (SeNPs) demonstrated low inhibitory effects (SA23).